C3 ELISA Kit

Catalog No. ABIN5706900

This Guinea Pig C3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Guinea Pig C3.

Quick Overview for C3 ELISA Kit (ABIN5706900)

Target: C3 (Complement Component 3 (C3))

Reactivity: Guinea Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details C3 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Analytical Method: Quantitative

Sensitivity: 0.64 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Background: Alternative name: ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling